【摘要】 目的 观察京尼平诱导胶原交联后猪眼巩膜生物力学的变化，为防治近视的发展探寻潜在的方法。方法 实验研究。离体猪眼45只，通过简单随机化方法分为9组，每组5眼。以0.01%、0.10%、1.00%京尼平（w/v）分别处理猪眼巩膜30 min；以核黄素/紫外线A处理的巩膜和未处理的巩膜作为对照组。处理后制作4.0 mm×10.0 mm的巩膜试件。24 h后用INSTRON万能材料机测试巩膜试件生物力学参数，另外还测试1.0%京尼平用药后1、6、12、24、36 h巩膜的生物力学参数；同时观察巩膜试件的颜色变化。应力和弹性模量比较采用方差分析。结果 与未治疗组比较，0.01%、0.10%、1.00%京尼平治疗24 h后应力提高了205%～498%，弹性模量增加了109%～467%。0.01%京尼平与核黄素/紫外线A具有相似的应力-应变曲线。而且在1.00%京尼平治疗后24 h内，随时间的延长，巩膜颜色逐渐变蓝；在治疗后1、6、12、24、36 h巩膜极限应力分别较未治疗巩膜增加了53%、146%、164%、223%和175%；弹性模量分别增加了80%、151%、185%、352%和296%。在京尼平治疗后24 h极限应力和弹性模量均达到峰值。结论 京尼平可以改善巩膜的生物力学性能，且疗效与其浓度有关。
【关键词】 生物力学； 巩膜； 胶原交联； 京尼平； 近视
DOI：10.3760/cma.j.issn.1674-845X.2014.05.004
基金项目：广东省科技计划项目（社会发展）（2007B031002001）
作者单位：510060 广州，中山大学中山眼科中心国家眼科重点实验室
通信作者：王铮，Email：wangzhengykzx@163.com
Change of biomechanical properties in porcine sclera treated with genipin
Liu Taixiang， Wu Junshu， Gu Yuwei， Yang Bin， Wang Zheng. Zhongshan Ophthalmic Center， Sun Yat-sen University， The State Key Laboratory of Ophthalmology， Guangzhou 510060， China
Corresponding author：Wang Zheng，Email：wangzhengykzx@163.com
【Abstract】 Objective Investigate the change of biomechanical properties in porcine sclera treated with genipin and to explore a potential method to prevent the progerssion of myopia. Methods Experimental study. Forty-five porcine cadaver eyes were randomly divided into 9 groups and each group had 5 eyes. Eyes were treated with genipin at concentrations （by w/v） of 0.01%， 0.10%， 1.00% for 30 min. Riboflavin/ultraviolet-A （UVA） treated and untreated samples were used as controls. After treatment， scleral strips of 4.0 mm×10.0 mm were cut. At 24 h after treatment， the stress-strain parameters of the strips were measured using a INSTRON 3343 biomaterial microtester. Additionally， we also performed the stress-strain test at 1， 6， 12， 24 and 36 h after treated with 1.00% genipin. Meanwhile the discoloration of sclera were observed and recorded with slit lamp camera system. The difference of the ultimate stress and modulus between treated and untreated groups were analyzed with One-Way ANOVA in SPSS 17.0. Results Compared with untreated groups， after treatment with genipin for 30 min， the ultimate stress and modulus were increased by 205% to 498% and 109% to 467% respectively. 0.01% genipin for 30min had a similar stress-strain curve with those of eyes treated with riboflavin/UVA. Within 24 h after treating with 1.00% genipin， the sclera exhibited a bluish color which became deeper with time. And at 1， 6， 12， 24 and 36 h after treatment， the ultimate stress were increased by 53%， 146%， 164%， 223% and 175% respectively. The modulus at 8% strain also increased by 80%， 151%， 185%， 352% and 296%. Conclusion Collagen crosslinking treated with genipin could increase the biomechanical strength in porcine sclera. The effect depends on the concentration of genipin.
【Key words】 Biomechanics； Sclera； Collagen crosslinking； Genipin； Myopia
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